LOCUS       40924_31635       13156 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pMpGWB236 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13156)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T.
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha
  JOURNAL   PLoS ONE 10 (9), E0138876 (2015)
  PUBMED    26406247
REFERENCE   2  (bases 1 to 13156)
  AUTHORS   Nishihama R, Ishizaki K, Kohchi T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2015) Contact:Ryuichi Nishihama Kyoto University, 
            Graduate School of Biostudies; Kitashirakawa-oiwake-cho, Sakyo-ku, 
            Kyoto, Kyoto 606-8502, Japan URL :http://www.lif.kyoto-u.ac.jp/e/
REFERENCE   3  (bases 1 to 13156)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13156)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2015"; volume: "10"; issue: "9"; pages: "E0138876"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-JUN-2015) Contact:Ryuichi Nishihama Kyoto University, Graduate 
            School of Biostudies"; volume: " Kitashirakawa-oiwake-cho, Sakyo-ku,
            Kyoto, Kyoto 606-8502, Japan URL :http"; pages: 
            "//www.lif.kyoto-u.ac.jp/e"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      307..2035
                     /label=MpEF1alpha promoter and 5'UTR
                     /note="MpEF1alpha promoter and 5'UTR"
                     /regulatory_class="promoter"
     CDS             2050..2763
                     /label=TagRFP-T
                     /note="photostable variant of TagRFP (Shaner et al., 2008)"
     protein_bind    2749..2873
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2910..2940
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2994..3650
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             3995..4297
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(4341..4465)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      4497..4749
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4783..4799)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4807..4823
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4831..4861)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4876..4897)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     polyA_signal    complement(4982..5154)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(5214..5744)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(5807..6483)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    complement(6678..6702)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7223..8011
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      8260..8848
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(9034..9174)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9518..9712)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9781..10851)
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(11283..11909)
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttcaaa tgagtcacac acattgttga gagacatatc aaaataactc aattgtaagg
      361 taagttgata gatttcccag ttaaatgcat tattttaaca atatttgtat ctcaaatttt
      421 caaaatcata tcaaatttgc agatcttgat gatgattcac aactctgtct tcgatttttg
      481 attgttaacg ggtatcgata tttgggtaac ttaaaaagaa tttgctaaag atcttaaaaa
      541 acagattaaa aaaacctttc aaaatagcta atcctttaaa tccgtttcac aatataatgc
      601 tcattgatga taatcgaaaa gattccattt taaagtgcat ataaattgct taattatctc
      661 tttctgtctc gtaatatttt gttctctcct attggatcgg acccgcatct tctcgatcag
      721 ttcactatac taatatttgg tcaacttcaa gagagattag aacttcgttc tccaaagtgt
      781 aaaggccgac aagcagaaaa ggaaacttca atcagaagcc ttctaggttt tcccgcgtcc
      841 caaaaagcga agggaggacg gggaggacgg tgaggccaga agaagaaagc aacagtaaaa
      901 tctcatgtga ttgcgtgcat gtcatggatc tatgggatgc tagtccaccg tttggaagta
      961 tgtgcagaag gagaaaggcg cgggaggcca aaagcgactt cggccacctc tctactttct
     1021 agaaagtttg ctcgcagctt gtaataagcg cggaaaaggt actcgaaaaa ccggagcaat
     1081 cggtgtggta tcagcagtcg ggttcgctaa acctacgtct tcttattttg gaatgttaaa
     1141 ttgaaagggc gtgccggccc taagcacgga cgatgtgaca gacggaggag tgtgagcaat
     1201 tcggatgacg atgctggccg gcagtcacga caaagagtcc gaaggggcat ggacagggca
     1261 atatataaat ggaaaagagg ctttgaatat tcctaaccct tcgtcgtcgg cctcccaact
     1321 tgccctcgca tgctgaattg gccgcctatc tgcgaatggc cggccgccga ccctaagtcg
     1381 caataacttg aacggagccg atgcaaagtc cttatcgaac gctcgttatt ataaacgggg
     1441 cagacctagg tatttaatta tatcaaaacc ctccgcaatt tcgctaatca ttccctaatt
     1501 tcttctactt tggcgtctag tgctgtggtc gcgcttgctc tcaaagctcg ctgggaggca
     1561 gtggagcgtc tggcttaaaa tcgatcgccg caaccctaga gttcctttca cttctttctc
     1621 tcttgcgtgg agagcatcga catcgaagag ctgcatcttg tcttctggta tgcgccttgt
     1681 gccctcgtct ctgtacgtcg ttacgattgc tcgatattgt gattgtctag acctgaaacc
     1741 cgactggagc ttttacgtgg tgtcgattgg ggcctcgagc gagtgatttt ttaggttcgt
     1801 tttagccaag tacatcccac tttggtcagt cctgttatct cggtaaggtg ttcagttcgg
     1861 tctctggtgc ggaggtgcct cgtaagtttc ctgcatgata gatttgtcta tccttgactt
     1921 gaagaggagt cgaaggtcag cgaggttggc tgtgtgctat ggacgtcggt gctccttggt
     1981 tccgtgcttc tatgggtgtt gcctcaacag tattgatgtg cctgcagaaa ggttgagctt
     2041 acaatgagcg agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg
     2101 aacaaccacc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
     2161 accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct
     2221 accagcttca tgtacggcag cagaaccttc atcaaccaca cccagggcat ccccgacttc
     2281 tttaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg
     2341 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc
     2401 aagatcagag gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacactcggc
     2461 tgggaggcca acaccgagat gctgtacccc gctgacggcg gcctggaagg cagaagcgac
     2521 atggccctga agctcgtggg cgggggccac ctgatctgca acttcaagac cacatacaga
     2581 tccaagaaac ccgctaagaa cctcaagatg cccggcgtct actatgtgga ccacagactg
     2641 gaaagaatca aggaggccga caaagagacc tacgtcgagc agcacgaggt ggctgtggcc
     2701 agatactgcg acctccctag caaactgggg cacaagctag agttatcaac aagtttgtac
     2761 aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg
     2821 cataaaaaac agactacata atactgtaaa acacaacata tccagtcact atggcggccc
     2881 ctcgaggggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
     2941 tgtggatttt gagttaggat ccggcgagat tttcaggagc taaggaagct aaaatggaga
     3001 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
     3061 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
     3121 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
     3181 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
     3241 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
     3301 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
     3361 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
     3421 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
     3481 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
     3541 tgctgatgcc gctggcgatt caggttcatc atgccgtctg tgatggcttc catgtcggca
     3601 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg
     3661 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
     3721 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtgtg ctatgaagca
     3781 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     3841 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     3901 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     3961 ggctcttttg ctgacgagaa cagggactgg tgaaatgcag tttaaggttt acacctataa
     4021 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     4081 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
     4141 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     4201 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     4261 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     4321 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
     4381 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     4441 gttcagcttt cttgtacaaa gtggttgata acagcgctta gagctcgaat ttccccgatc
     4501 gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga
     4561 ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga
     4621 cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga
     4681 tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt
     4741 tactagatcg ggaattggtt ccggaaccaa ttcgtaatca tggtcatagc tgtttcctgt
     4801 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     4861 agcctggggt gcctaatgag tgagctaact cacattaggc tgaattaggc gcgcctattt
     4921 ctgaattcaa cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg
     4981 ggatctggat tttagtactg gattttggtt ttaggaatta gattttattg atagaagtat
     5041 tttacaaata caaatacata ctaagggttt cttatatgct caacacatga gcgaaaccct
     5101 ataggaaccc taattccctt atctgggaac tactcacaca ttattatgga gaaactcgag
     5161 cttgtcgatc gactctaggc cgggaagccg atctcggctt gaacgaattg ttaggtggcg
     5221 gtacttgggt cgatatcaaa gtgcatcact tcttcccgta tgcccaactt tgtatagaga
     5281 gccactgcgg gatcgtcacc gtaatctgct tgcacgtaga tcacataagc accaagcgcg
     5341 ttggcctcat gcttgaggag attgatgagc gcggtggcaa tgccctgcct ccggtgctcg
     5401 ccggagactg cgagatcata gatatagatc tcactacgcg gctgctcaaa cttgggcaga
     5461 acgtaagccg cgagagcgcc aacaaccgct tcttggtcga aggcagcaag cgcgatgaat
     5521 gtcttactac ggagcaagtt cccgaggtaa tcggagtccg gctgatgttg ggagtaggtg
     5581 gctacgtctc cgaactcacg accgaaaaga tcaagagcag cccgcatgga tttgacttgg
     5641 tcagggccga gcctacatgt gcgaatgatg cccatacttg agccacctaa ctttgtttta
     5701 gggcgactgc cctgctgcgt aacatcgttg ctgctgcgta ccatggtcga tcgacagatc
     5761 tgcgaaagct cgagagagat agatttgtag agagagactg gtgatttcag cgtgtcctct
     5821 ccaaatgaaa tgaacttcct tatatagagg aagggtcttg cgaaggatag tgggattgtg
     5881 cgtcatccct tacgtcagtg gagatatcac atcaatccac ttgctttgaa gacgtggttg
     5941 gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt
     6001 cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc
     6061 caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga
     6121 ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga
     6181 ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttcacatcaa
     6241 tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt
     6301 gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta
     6361 tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga
     6421 cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc
     6481 tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg
     6541 tcgtgctcca ccatgttggc aagctgctct agccaatacg caaaccgaat tcagaaataa
     6601 attcagccta attcggcgtt aattcagtac attaaaaacg tccgcaatgt gttattaagt
     6661 tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc
     6721 cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac
     6781 ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc
     6841 ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat
     6901 gttgattgta acgatgacag agcgttgctg cctgtgatca attcgggcac gaacccagtg
     6961 gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg
     7021 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     7081 ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
     7141 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg
     7201 ccctaaaaca aagttaaaca tcatggggga agcggtgatc gccgaagtat cgactcaact
     7261 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
     7321 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac
     7381 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac
     7441 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca
     7501 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca
     7561 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat
     7621 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact
     7681 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg
     7741 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
     7801 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat
     7861 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg
     7921 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt
     7981 gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta gctagaaatt cgttcaagcc
     8041 gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg cactaagcac ataattgctc
     8101 acagccaaac tatcaggtca agtctgcttt tattattttt aagcgtgcat aataagccct
     8161 acacaaattg ggagatatat catgcatgac caaaatccct taacgtgagt tttcgttcca
     8221 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     8281 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     8341 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     8401 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     8461 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     8521 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     8581 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     8641 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     8701 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     8761 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     8821 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     8881 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     8941 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     9001 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     9061 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     9121 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     9181 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     9241 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     9301 aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc
     9361 ttgtccgcgc cctggtagat tgcctggccg taggccagcc atttttgagc ggccagcggc
     9421 cgcgataggc cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc
     9481 tttttgcagc tcttcggctg tgcgctggcc agacagttat gcacaggcca ggcgggtttt
     9541 aagagtttta ataagtttta aagagtttta ggcggaaaaa tcgccttttt tctcttttat
     9601 atcagtcact tacatgtgtg accggttccc aatgtacggc tttgggttcc caatgtacgg
     9661 gttccggttc ccaatgtacg gctttgggtt cccaatgtac gtgctatcca caggaaagag
     9721 accttttcga cctttttccc ctgctagggc aatttgccct agcatctgct ccgtacatta
     9781 ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag cgcatgacta ggatcgggcc
     9841 agcctgcccc gcctcctcct tcaaatcgta ctccggcagg tcatttgacc cgatcagctt
     9901 gcgcacggtg aaacagaact tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt
     9961 cttgaacaac catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa
    10021 acggccgatg ccgggatcga tcaaaaagta atcggggtga accgtcagca cgtccgggtt
    10081 cttgccttct gtgatctcgc ggtacatcca atcagctagc tcgatctcga tgtactccgg
    10141 ccgcccggtt tcgctcttta cgatcttgta gcggctaatc aaggcttcac cctcggatac
    10201 cgtcaccagg cggccgttct tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt
    10261 taaccgaatg caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca
    10321 gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc
    10381 ttcccggtat cggttcatgg attcggttag atgggaaacc gccatcagta ccaggtcgta
    10441 atcccacaca ctggccatgc cggccggccc tgcggaaacc tctacgtgcc cgtctggaag
    10501 ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc gacagacgga aaacggccac
    10561 gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc
    10621 tggttgctcg tcgcccttgg gcggcttcct aatcgacggc gcaccggctg ccggcggttg
    10681 ccgggattct ttgcggattc gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc
    10741 tgcctcgatg cgttgccgct gggcggcctg cgcggccttc aacttctcca ccaggtcatc
    10801 acccagcgcc gcgccgattt gtaccgggcc ggatggtttg cgaccgctca cgccgattcc
    10861 tcgggcttgg gggttccagt gccattgcag ggccggcaga caacccagcc gcttacgcct
    10921 ggccaaccgc ccgttcctcc acacatgggg cattccacgg cgtcggtgcc tggttgttct
    10981 tgattttcca tgccgcctcc tttagccgct aaaattcatc tactcattta ttcatttgct
    11041 catttactct ggtagctgcg cgatgtattc agatagcagc tcggtaatgg tcttgccttg
    11101 gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc ggcaactgaa agttgacccg
    11161 cttcatggct ggcgtgtctg ccaggctggc caacgttgca gccttgctgc tgcgtgcgct
    11221 cggacggccg gcacttagcg tgtttgtgct tttgctcatt ttctctttac ctcattaact
    11281 caaatgagtt ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc
    11341 tcgggttctg attcaagaac ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc
    11401 tgcgtgatac gggactcaag aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca
    11461 ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg tagccttcca
    11521 tccgtgacct caatgcgctg cttaaccagc tccaccaggt cggcggtggc ccatatgtcg
    11581 taagggcttg gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc ggacacagcc
    11641 aagtccgccg cctggggcgc tccgtcgatc actacgaagt cgcgccggcc gatggccttc
    11701 acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc
    11761 acggccgccc aatcgcgggc actgccctgg ggatcggaat cgactaacag aacatcggcc
    11821 ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct
    11881 ttctggttaa gtacagcgat aaccttcatg cgttcccctt gcgtatttgt ttatttactc
    11941 atcgcatcat atacgcagcg accgcatgac gcaagctgtt ttactcaaat acacatcacc
    12001 tttttagacg gcggcgctcg gtttcttcag cggccaagct ggccggccag gccgccagct
    12061 tggcatcaga caaaccggcc aggatttcat gcagccgcac ggttgagacg tgcgcgggcg
    12121 gctcgaacac gtacccggcc gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg
    12181 gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt cattctcggc
    12241 ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc gccgcctggc
    12301 ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg agcgatgcac
    12361 gccaagcagt gcagccgcct ctttcacggt gcggccttcc tggtcgatca gctcgcgggc
    12421 gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca aacttcacgc ctcgggcctt
    12481 ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg gaacgctcga actcggcaat
    12541 gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg cccacggctc
    12601 tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt ccagtaggtc
    12661 gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc cagggcgtag
    12721 gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg gtgccggtga tcttctcgga
    12781 aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca agtcctggtc
    12841 gtcggtgctg acgcgggcat agcccagcag gccagcggcg gcgctcttgt tcatggcgta
    12901 atgtctccgg ttctagtcgc aagtattcta ctttatgcga ctaaaacacg cgacaagaaa
    12961 acgccaggaa aagggcaggg cggcagcctg tcgcgtaact taggacttgt gcgacatgtc
    13021 gttttcagaa gacggctgca ctgaacgtca gaagccgact gcactatagc agcggagggg
    13081 ttggatcaaa gtactttgat cccgagggga accctgtggt tggcatgcac atacaaatgg
    13141 acgaacggat aaacct
//
